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(USP) LC Methods

Home >> (USP) LC Methods

USP Designations

The US Pharmacopeia (USP) is a standard source for many pharmaceutical methods that specifies columns by packing material rather than by manufacturer. Listed below are the recommended Agilent Technologies HPLC columns suitable for most LC methods listed with the USP.

USP Method USP Packing Materials Column Particle Size(pm)
L1 Octadecyl Silane chemically bonded to porous silica or
ceramic micro-particles, 1.5 to 10 pm in diameter
Poroshell 120 EC- C18
Poroshell 120 SB- C18
ZORBAX Eclipse Plus C18
ZORBAX Eclipse XDB-C18
ZORBAX SB-C18
ZORBAX Rx-C18
ZORBAX Extend-C18
ZORBAX ODS
ZORBAX ODS classic
Pursuit XRs C18
Pursuit C18
Polaris C18-A
Polaris C18-Ether
Sep Tech ST60 C18
Sep Tech ST150 C18
2.7
2.7
1.8,3.5,5
1.8,3.5,5,7
1.8,3.5,5,7
3.5,5
1.8,3.5,5,7
3.5,5,7
5
3,5,10
3,5,10
3,5,10
3,5
10
10
L2 Octadecyl Silane chemically bonded to porous silica gel
of a controlled surface porosity that has been bonded
to a solid spherical core, 30 to 50 pm in diameter
N/A  
L3 Porous silica particles, 5 to 10
pm in diameter
ZORBAX SIL
ZORBAX Rx-Sil
Pursuit XRs Si
Polaris Si-A
MicroSpher Si
Microsorb 100 Si
5
3.5,5
3,5,10
5,10
5
5
L4 Silica get of controlled surface porosity bonded to a solid spherical core, 30 to 50 pm in diameter    
L5 Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 pm in diameter N/A  
L6 Strong cation-exchange packing: sulfonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 pm in diameter N/A  
L7 Octyl Silane chemically bonded to totally porous
microsilica particles, 1.5 to 10 pm in diameter
Poroshell 120 EC-C8
ZORBAX Eclipse Plus C8
ZORBAX Eclipse XDB-C8
ZORBAX SB-C8
ZORBAX RX-C8
ZORBAX C8
Pursuit XRs C8
Pursuit C8
Polaris C8-A
Polaris C8-Ether
Microsorb 100 C8
2.7
1.8,3.5,5
1.8,3.5,5,7
1.8,3.5,5,7
1.8,3.5,5,7
5
3,5,10
3,5,10
3,5
3,5
5
L8 An essentially monomolecular  layer of
aminopropylsilane chemically bonded to totally porous silica gel support, 10 pm in diameter 
ZORBAX NH2
Polaris NH2
Microsorb 100 Amino
5
5
5
L9 10 pm irregular, totally porous silica gel having a chemically bonded, strongly acidic cation
exchange coating
ZORBAX SCX 5 spherical
L10 Nitrile groups chemically
bonded to porous silica particles, 3 to 10 pm in diameter
ZORBAX CN
ZORBAX SB-CN
ZORBAX Eclipse XDB-CN
Microsorb 100 Cyano
5
3.5,5
3.5,5
5
L13 Trimethylsilane chemically
bonded to porous silica particles, 3 to 10 pm in diameter
ZORBAX TMS 5
L14 Silica gel 10 pm in diameter
with a chemically bonded, strongly basic quaternary ammonium anion exchange coating
ZORBAX SAX
IonoSpher A
5
 
L15 Heryl silane chemically bonded to totally porous silica particles, 3 to 10 pm in diameter MetaSil C6  
L16 Dimethyl silane chemically bonded to totally porous
silica particles, 3 to 10 pm in diameter
N/A  
L17 Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 pm in diameter Hi-Plex H 8
  Amino and cyano groups chemically bounded to porous silica particles, 5 to 10 pm in diameter N/A  
L19 Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 9 pm in diameter
Hi-Plex Ca
Hi-Plex Ca (Duo)
8
8
L20 Dihydroxypropane groups chemically bonded to porous silica particles, 3 to 10 pm in diameter LiChrospher Diol 5
L21 A rigid, spherical styrene divinylbenzene copolymer,
5 to 10 pm in diameter
PLgel
PLRP-S 100A
PLRP-S 300A
PLRP-S 1000A
3,5,10,20
3,5,8
3,5,8
5,8
L22 A cation exchange resign made of porous polystyrene
gel wioth sulfonic acid groups, about 10 pm in size
Hi-Plex H 8
L23 An ion exchange resign made of porous
polymethacrylate or polyacrylate gel with quaternary ammonia groups, about 10 pm in size.
N/A  
L24 A semi-rigid hydrophilic gel consisting of  vinyl
polymers with numerous hydroxyl group on the matrix surface, 32 to 63 pm in diameter  
N/A  
L25 Packing having the capacity to separate compounds with
a MW range from 1,000 to 5,000 da (as determined
by the polyethylene oxide), applied to neutral, ionic and cationic water-soluble polymers
PL aquagel-OH 5,8
L26 Butyl silane chemically bounded to porous silica
particles, 5 to 10 pm in diameter
MicroSorb C4 5
L27 Porous silica particles, 30 to 50 pm in diameter

Bondesil Silica

 
L28 A multifunctional support, which consists of a high
purify, 100 A, spherical silica substrate that has been bonded with anionic (amine) functionality in addition to conventional reversed-phase C8 functionality
N/A  
L29 Gamma alumina, reversed phase, low carbon percentage by weight, alumina based polybutadiene spherical
particles, 5 pm diameter with a pore diameter of 80A
N/A  
L30 Ethyl silane chemically bonded to a totally porous
silica particles, 3 to 10 pm in diameter
N/A  
L31 A strong anion-exchange resin-quaternary amine
bonded on latex particles attached to a core of 8.5 pm macroporous particles having a pore size of 2000A
and consisting of ethylvinylbenzene cross-linked
with 55% divinylbenzene
N/A  
L32 A chiral ligand-exchange packing L- praline copper
complex covalently bonded to irregularly shaped silica particles, 5 to 10 pm in diameter
N/A  
L33 Packing having the capacity to separate proteins
by molecular size over a range of 4,000 to 400,000 da.
It is spherical, silica-based, and processed to provide pH stability
ZORBAX GF-250
Bio SEC-3
Bio SEC-5
4
3
5
L34 Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 9 pm in diameter Hi-Plex  Pb 8
L36 A zirconium stabilized Spherical Silica Packing with a hydrophilic (diol – Type) molecular monolayer bonded phase
ZORBAX GF – 250
ZORBAX GF – 450
4
6
L37 Polymethcrylate get packing having the capacity to separate proteins by molecular size over a range  of
2,000 to 4,000 da MW
N/A  
L39 Hydrophilic Polyhydroxymethacrylate Porous spherical resin    N/A  
L40 Cellulose tris-3.5, dimethylphenlycarbamate coated
porous silica particles, 5 to 20 pm in diameter 
N/A  
L41 Immobilized alpha – acid glyci-protein on spherical
silica particles, 5pm in diameter
N/A  
L42 Octylsilane and octadecylsilane groups chemically bonded to porous silica particles N/A  
L43 Pentafluorophenyl groups chemically bonded to silica particles 5 to 10 pm in diameter  Pursuit PFP 3.5
L44 A Multifunctional support, which consists of a high
purity, 60A spherical silica substrate, that has been
bonded with a cationic exchanger, sulfonic acid
functionality in addition to a conventional
reversed phase C8 functionality
N/A  
L45 Beta cyclodextrin bonded to porous silica particles,
5 to 10 pm in diameter
ChiraDex Chiral 5
L46 Polystyrene/divinylbenzene substrate agglomerated
with quaternary amine functionalized latex beads,
10 pm in diameter
N/A  
L47 High capacity anion exchange microporous substrate,
fully functionalized with a trimethyl-amine group,
8 pm in diameter
N/A  
L48 Sulfonated, cross-linked polystyrene with an outer layer
of submicron, porous, anion-exchange microbeads,
15 pm in diameter
N/A  
L49 Amylose tris-3,5-dimethylphenyl-carabamate-coated, porous, spherical, silica particles, 5 to 10
pm in diameter
N/A  
L50 A strong cation exchange resin made of porous silica
with sulfopropyl groups, 5 to 10 pm in diameter
ZORBAX 300SCX 5
L51 A reversed-phase packing made by coating a thin
layer of polybutadiene on to spherical porous zirconia particles, 3 to 10 pm in diameter
N/A  
L52 Multifunction resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists
of ethylvinyl-benzene, 55% cross-linked with divinyl-benzene copolymer, 3 to 15 pm in diameter, and a
surface area of not less than 350m2/g, substrate is
coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene.
N/A  
L53 An anion-exchange resin consisting of rigid, spherical styrene-divinylbenzene copolymer with trimethylammonium group at a loading of about 2
meq per g, 3 to 29 pm in diameter  
Bio SAX 3,5,10
L54 Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, 7 to 11 pm in diameter N/A  
L55 Weak cation exchange resin consisting of  ethylvinylbenzene, 55%  cross-linked with divinylbenzene copolymer, 3 to 15 pm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500 peq/column N/A  
L56 Propyl silane chemically bonded to totally porous silica particles, 3 to 10 pm in diameter SB-C3 3,5
L57 A chiral-recognition protein, ovomucoid, chemically
bonded to silica particles, about 5 pm in diameter,
with a pore size of 120 angstroms
Ultron ES-OVM 5
L58 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 pm in diameter
Hi-Plex Na
Hi-Plex Na (Octo)
10
8
L59 Packing having the capacity to separate proteins by molecular weight over the range of 5 to 7000 kDa. It is spherical (5-10 pm), silica-based, and processed to
provide hydrophilic characteristics and pH stability
N/A  
L60 Spherical, porous silica gel, 10 pm or less in diameter,
the surface of which has been covalently modified with alkyl amide groups and endcapped
Bonus-RP
Polaris Amide-C18
1.8,3.5,5
3,5